WebArticle Snippet: The manufacturer’s protocol for PMA treatment was used and involved the use of the PMA-Lite™ LED Photolysis Device (Biotium, Fremont, CA, USA). Techniques: … WebApr 30, 2024 · PMA-free samples served as controls for each condition tested. Tubes were covered with aluminium foil and shaken on an orbital shaker for 5, 10, 20, 30 or 70 minutes. Samples were then exposed to light using the PMA-Lite LED Photolysis Device (Cambridge BioSciences, Cambridge, UK) for 5, 10, 20, 30 or 40 minutes.
PMA-Lite™ LED Photolysis Device - Biotium - Cambridge …
WebThe PMA-Lite™ LED Photolysis Device can be used to provide continuous illumination for up to 18 samples at a time, for convenient and controlled photoactivation of PMA, … WebJan 21, 2024 · PMA, a fluorescent dye that indicates cell membrane impairment, can only enter bacteria with damaged or compromised membranes. Upon photolysis with LED light, PMA binds covalently to DNA and prevents DNA amplification by PCR. Thus, only live bacterial DNA can be amplified by PMA-qPCR. how do worms contribute to soil improvement
Detection and identification of potentially infectious ... - Nature
WebThe PMA-Lite LED Photolysis Device can be used to provide continuous illumination for up to 18 samples at a time, for convenient and controlled photoactivation of PMA, PMAxx, … WebPMA-Lite™ LED Photolysis Device PMA-Lite™ is specifically designed for photoactivation of PMA™ and other similar dyes. Receive a free vial of PMA™ dye when you purchase a … WebPMA-Lite™ 2.0 LIGHT Photolysis Device; View all in Fittings; Cushions, Appurtenances, & Background Quenchers. Back; Overview of TrueBlack® Backgrounds Reducers; Blocking Buffers & Background Quenchers; ... PMA-Lite™ 2.0 LED Photolysis Contrivance; View all in Equipment; BACK. Nucleic Acid Liquid Stains how do worm composters work