Fastpfu buffer
WebJul 5, 2024 · Methods: In this study, we systematically described the characteristics of bacteria in the oral and nasal niches in healthy children, CP children, healthy adolescents, CP adolescents, and postoperative adolescents by 454-pyrosequencing technology (V3–V6) to determine bacterial differences induced by CP. WebApr 28, 2024 · The raw materials used for PCR reaction were as follows: Transgen AP221–02:Transstart FastPFU DNA Polymerase, 20μL reaction system: 5 × TransStart FastPfu buffer (4μL), 2.5 mM dNTPs (2μL), forward primer (5μM) 0.8μL, reverse primer (5μM) 0.8μL, TransStart FastPfu polymerase 0.4μL,BSA 0.2μL, template DNA 10 ng, up …
Fastpfu buffer
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Webmixtures containing 4 lL of 5X FastPfu Buffer, 2 lL of 2.5 mM dNTPs, 0.8 lL of each primer (5 lM), 0.4 lL of FastPfu Polymerase, and 10 ng of template DNA. The PCR products were extracted from a 2% agarose gel and further purified using an AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA). The products were quantified using WebJan 30, 2024 · DNA samples were amplified in triplicate on an ABI GeneAmp ® 9700 PCR System (ABI, MA, USA) in PCR reactions containing 2 μl 2.5 mM dNTPs, 0.4 μl FastPfu Polymerase (Transgen Biotech, Beijing ...
WebSep 21, 2024 · The PCR reactions were conducted using the following program: 3 min of denaturation at 95°C, 27 cycles of 30 s at 95°C, 30 s for annealing at 55°C, and 45 s for elongation at 72°C, and a final extension at 72°C for 10 min. PCR reactions were performed in a triplicate 20 μL mixture containing 4 μL of 5 × FastPfu Buffer, 2 μL of 2.5 mM ... WebFastPfu FLY Sets Higher Standards of High-Fidelity DNA Polymerase Performance. Whereas Phusion and Q5 failed, FastPfu FLY succeeded easily at... 1-877-661-4711 …
WebApr 14, 2024 · Twenty-microliter bacteria/fungi mixtures for PCR contained 4 μL of 5× TransStart FastPfu buffer/2 μL of 10× Buffer, 2.5 mM dNTPs in 2 μL, 0.8 μL of forward primer (5 μM), 0.8 μL of reverse primer (5 μM), 0.4 μL of FastPfu Polymerase/0.2 μL of rTaq Polymerase, 0.2 μL of BSA, 10 ng of template DNA, and finally ddH2O up to 20 μL, with … WebA: In a 10 µl reaction as follows: add purified PCR product, 0.2 μl of Taq DNA Polymerase, 0.5 μl of 10 mM dNTP (or 0.5 μl of 2.5 mM dATP) and 1 μl of 10xTaq Buffer. Incubate at 72°C for 5–15 minutes. TransFast ® Taq DNA Polymerase EasyTaq ® DNA Polymerase EasyTaq ® DNA Polymerase for PAGE TransTaq ®-T DNA Polymerase
WebMar 8, 2024 · We then collected below-ground root biomass to a depth of 30 cm using soil cores (diameter 7 cm) in each of these five quadrats as well. Roots were collected by …
Web本发明公开一种基于环境DNA技术监测淡水底栖动物群落多样性的方法，包括步骤：(1)采集表层水样；(2)水样过滤与eDNA提取；(3)PCR扩增；(4)高通量测序，获得OTU的代表序列；(5)底栖动物比对数据库的建立；(6)比对建立的底栖动物数据库，对待测样品OTUs代表性序列进行物种注释。本发明取样简单、人力 ... hot def scrabbleWeb侯 磊，薛 蓓，薛会英 (西藏农牧学院，西藏 林芝 860000) 土壤线虫作为土壤动物中的优势类群，是草地生态系统中的消费者，关系到草地生产力的增减[1]。 pt. osell selection indonesiaWebEach batch of TransStart® FastPfu DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA. Restrictions For … pt. opelon garment indonesiaWebProduct Details. TransStart® FastPfu DNA Polymerase is a fast, high fidelity and high processivity hot start DNA polymerase. • Extension rate is about 2-4 kb/min. • … hot demon boy minecraft skinsWebMar 11, 2024 · A 20-μL PCR reaction system contained 4 μL of 5 × FastPfu Buffer, 2 μL of 2.5 mM dNTPs, 0.8 μL each of the 338F and 806R primers, 0.4 μL of FastPfu polymerase, 10 ng of template DNA, 0.2 μL of BSA, and residual ddH 2 O. pt. oryx servicesWebDec 14, 2024 · The PCR mixtures contained 5 × TransStart FastPfu buffer 4 μL, 2.5 mM dNTPs 2 μL, forward primer (5 μM) 0.8 μL, reverse primer (5 μM) 0.8 μL, TransStart FastPfu DNA Polymerase 0.4 μL, template DNA 10 ng, and, finally, ddH 2 O up to 20 μL. PCR assays were performed in triplicate. pt. oto multiarthaWebMay 1, 2024 · Fresh soil samples were divided into two subsamples, one of which was stored at 4 °C before determination of physicochemical properties, and the other was … pt. pacinesia chemical industry